El MIT prueba secreto de la dureza del huesoContributed by: AdminStaff1 · Views: 1,281
Contributed by: AdminStaff1 · September 07, 2007 @ 06:17 AM MDT · Views: 1,281
MIT probes secret of bone's strengthDenise Brehm, Civil and Environmental Engineering
New research at MIT has revealed for the first time the role of bone's atomistic structure in a toughening mechanism that incorporates two theories previously proposed by researchers eager to understand the secret behind the material's lightweight strength.
Photo / Donna Coveney
MIT Professor Markus Buehler has helped reveal why bones are so tough. The object on the screen is a triple helical tropocollagen molecule, a fundamental building block of bone. Next to the molecule are nanosized hydroxyapatite chalk-like crystals. In his work he simulates the behavior of the composite of tropocollagen and hydroxyapatite during deformation.
"The newly discovered molecular mechanism unifies controversial attempts of explaining sources of the toughness of bone, because it illustrates that two of the earlier explanations play key roles at the atomistic scale," said the study's author, Esther and Harold E. Edgerton Professor Markus Buehler of MIT's Department of Civil and Environmental Engineering. "It's quite possible that each scale of bone--from the molecular on up--has its own toughening mechanism," said Buehler. "This hierarchical distribution of toughening may be critical to explaining the intriguing properties of bone and laying the foundation for new materials design that includes the nanostructure as a specific design variable."
Unlike synthetic building materials, which tend to be homogenous throughout, bone is heterogeneous living tissue whose cells undergo constant change. Scientists have classified bone's basic structure into a hierarchy of seven levels of increasing scale. Level 1 bone consists of bone's two primary components: chalk-like hydroxyapatite and collagen fibrils, which are strands of tough, chewy proteins. Level 2 bone comprises a merging of these two into mineralized collagen fibrils that are much stronger than the collagen fibrils alone. The hierarchical structure continues in this way through increasingly larger combinations of the two basic materials until reaching level 7, or whole bone.
Buehler scaled down his model to the atomistic level, to see how the molecules fit together--and equally important for materials scientists and engineers--how and when they break apart. More precisely, he looked at how the chemical bonds within and between molecules respond to force. Last year, he analyzed for the first time the characteristic staggered molecular structure of collagen fibrils, the precursor to level 1 bone.
In his newer research, he studied the molecular structure of the mineralized collagen fibrils that make up level 2 bone, hoping to find the mechanism behind bone's strength, which is considerable for such a lightweight, porous material.
At the molecular level, the mineralized collagen fibrils are made up of strings of alternating collagen molecules and consistently sized hydroxyapatite crystals. These strings are "stacked" together in a staggered fashion such that the crystals appear in stair-step configurations. Weak bonds form between the crystals and molecules in the strings and between the strings.
Image / Markus Buehler
The top left image shows the mineralized collagen fibrils of bone with the characteristic stair-step configuration of hydroxyapatite crystals (represented in yellow) and collagen molecules (purple). At bottom, each brick-like structure represents a mineralized collagen fibril within the level 2 bone fabric
Previously, some researchers suggested that the fundamental key to bone's toughness is the "molecular slip" mechanism that allows weak bonds to break and "stretch" the fabric without destroying it. Others have cited the characteristic length of bone's hydroxyapatite crystals (a few nanometers) as an explanation for bone's toughness; the crystals are too small to break easily.
At the atomistic scale, Buehler sees the interplay of both these mechanisms. This suggests that competing explanations may be correct; bone relies on different toughening mechanisms at different scales.
Buehler also discovered something very notable about bone's ability to tolerate gaps in the stretched fibril fabric. These gaps are of the same magnitude--several hundred micrometers--as the basic multicellular units or BMUs associated with bone's remodeling. BMUs are a combination of cells that work together like a small boring tool that eats away old bone at one end and replaces it at the other, forming small crack-like cavities in between as it works its way through the tissue.
Thus, the mechanism responsible for bone's strength at the molecular scale also explains how bone can remain so strong--even though it contains those many tiny cracks required for its renewal.
Image / Markus Buehler
This image demonstrates that the weak bonds between collagen molecules and hydroxyapatite crystals break, leaving small gaps in the fabric without rending it entirely.
"Engineers typically over-dimension structures in order to make them robust. Nature creates robustness by hierarchical structures," said Buehler.
This work was funded by a National Science Foundation CAREER award and a grant from the Army Research Office.
Courtesy: MIT news office